smad1  (Proteintech)

Journal: Frontiers in Oncology

Article Title: Chorordin-like 1 inhibits pancreatic cancer cell migration and invasion: involvement of the BMP4/SMAD pathway

doi: 10.3389/fonc.2025.1633464

Figure Lengend Snippet: Overexpression of CHRDL1 inhibits BMP4-Induced SMAD phosphorylation in pancreatic cancer cells. (A, B) p-SMAD 1/5/9, t-SMAD 1/5/9, RUNX2 and CHRDL1 protein levels in PANC-1 cells were detected using Western blots. The immunoblots were calculated by densitometric analysis using GAPDH as the internal reference. (C, D) p-SMAD 1/5/9, t-SMAD 1/5/9, RUNX2 and CHRDL1 protein levels in SW1990 cells were detected using Western blots. The immunoblots were calculated by densitometric analysis using GAPDH as the internal reference. n = 6 in each group, * P < 0.05 vs. OE-NC, # P < 0.05 rhBMP4+OE-NC, one-way ANOVA followed by Sidak’s multiple comparisons test for multi-group.

Article Snippet: The antibodies used for Western blot detection were as follows: p-SMAD 1/5/9 (1:1,1000, Cell Signaling Technology, MA, USA, #9516), SMAD 1/5/9 (1:500, Cell Signaling Technology, MA, USA, #12656), RUNX2 (1:1,1000, Abcam, Cambridge, UK, ab192256), CHRDL1 (1:1,1000, Abcam, Cambridge, UK, ab227473), GAPDH (1:20,000, Proteintech, Wuhan, China, 60004-1-Ig).

Techniques: Over Expression, Phospho-proteomics, Western Blot